Immunohistochemistry:Article Title: CLEC12A signaling represses protective immune responses and contributes to hippocampal pathology in neurotropic picornavirus infection
Article Snippet: .. Details of antibodies used for immunohistochemistry Primary antibody/dilution Supplier (clone/catalogue number) Pre-treatment Blocking solution Secondary antibody Specificity CD107b 1:200 (mc) AbD Serotec (MCA2293) 20 minutes citrate buffer/MW Rabbit Rabbit anti-rat Macrophages/microglia CD3 1:500(pc) DakoCytomation (A0452) 20 minutes citrate buffer/MW Goat Goat-anti-rabbit T cells CD4 1:2000 (mc) BD Pharmingen, (550280) - Rabbit Rabbit anti-rat CD4+ T cells CD8 1:1000 (mc) BD Pharmingen, (553038) - Rabbit Rabbit anti-rat CD8+ T cells CD45R 1:1000 (biotinylated mc) BD Biosciences (553085) 20 minutes citrate buffer/MW - - B cells GFAP 1:1000 (pc) DakoCytomation (Z0334) - Goat Goat anti-rabbit Astrocytes Granzyme B 1:400 (pc) Abcam (ab4059) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Effector T cells and NK cells NeuN 1:1600 (mc) Merck/Millipore (MAB377) 20 minutes citrate buffer/MW Goat Goat anti-mouse Neurons TMEV 1:2000 (pc) Department of Pathology, University of Veterinary Medicine Hannover, Germany* - Goat Goat anti-rabbit TMEV capsid protein VP1 β-APP 1:2000 (mc) Merck/Millipore (MAB348) 20 minutes citrate buffer/MW Goat Goat anti-mouse Axonal damage TNF-α 1:50 (mc) Novus NBP2-34303) 20 minutes citrate buffer/MW Goat Goat anti-mouse Inflammatory cytokines producing cells CD11c 1:500 (pc) Invitrogen (PA5-79537) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Activated microglia and infiltrating myeloid or dendritic cells MHC-I 1:200 (pc) Biorbyt (Orb5449) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Presentation of endogenous peptides to CD8+ T cell β-APP = beta-amyloid precursor protein; GFAP = glial fibrillary acidic protein; mc = monoclonal; MW = microwave oven; NeuN = neuronal nuclei; pc = polyclonal; TMEV = Theiler’s murine encephalomyelitis virus; TNF-α = tumor necrosis factor-α; NK cells = natural killer cells; *Kummerfeld et al. [88] Supplementary Table S2. ..
Blocking Assay:Article Title: CLEC12A signaling represses protective immune responses and contributes to hippocampal pathology in neurotropic picornavirus infection
Article Snippet: .. Details of antibodies used for immunohistochemistry Primary antibody/dilution Supplier (clone/catalogue number) Pre-treatment Blocking solution Secondary antibody Specificity CD107b 1:200 (mc) AbD Serotec (MCA2293) 20 minutes citrate buffer/MW Rabbit Rabbit anti-rat Macrophages/microglia CD3 1:500(pc) DakoCytomation (A0452) 20 minutes citrate buffer/MW Goat Goat-anti-rabbit T cells CD4 1:2000 (mc) BD Pharmingen, (550280) - Rabbit Rabbit anti-rat CD4+ T cells CD8 1:1000 (mc) BD Pharmingen, (553038) - Rabbit Rabbit anti-rat CD8+ T cells CD45R 1:1000 (biotinylated mc) BD Biosciences (553085) 20 minutes citrate buffer/MW - - B cells GFAP 1:1000 (pc) DakoCytomation (Z0334) - Goat Goat anti-rabbit Astrocytes Granzyme B 1:400 (pc) Abcam (ab4059) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Effector T cells and NK cells NeuN 1:1600 (mc) Merck/Millipore (MAB377) 20 minutes citrate buffer/MW Goat Goat anti-mouse Neurons TMEV 1:2000 (pc) Department of Pathology, University of Veterinary Medicine Hannover, Germany* - Goat Goat anti-rabbit TMEV capsid protein VP1 β-APP 1:2000 (mc) Merck/Millipore (MAB348) 20 minutes citrate buffer/MW Goat Goat anti-mouse Axonal damage TNF-α 1:50 (mc) Novus NBP2-34303) 20 minutes citrate buffer/MW Goat Goat anti-mouse Inflammatory cytokines producing cells CD11c 1:500 (pc) Invitrogen (PA5-79537) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Activated microglia and infiltrating myeloid or dendritic cells MHC-I 1:200 (pc) Biorbyt (Orb5449) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Presentation of endogenous peptides to CD8+ T cell β-APP = beta-amyloid precursor protein; GFAP = glial fibrillary acidic protein; mc = monoclonal; MW = microwave oven; NeuN = neuronal nuclei; pc = polyclonal; TMEV = Theiler’s murine encephalomyelitis virus; TNF-α = tumor necrosis factor-α; NK cells = natural killer cells; *Kummerfeld et al. [88] Supplementary Table S2. ..
Immunopeptidomics:Article Title: CLEC12A signaling represses protective immune responses and contributes to hippocampal pathology in neurotropic picornavirus infection
Article Snippet: .. Details of antibodies used for immunohistochemistry Primary antibody/dilution Supplier (clone/catalogue number) Pre-treatment Blocking solution Secondary antibody Specificity CD107b 1:200 (mc) AbD Serotec (MCA2293) 20 minutes citrate buffer/MW Rabbit Rabbit anti-rat Macrophages/microglia CD3 1:500(pc) DakoCytomation (A0452) 20 minutes citrate buffer/MW Goat Goat-anti-rabbit T cells CD4 1:2000 (mc) BD Pharmingen, (550280) - Rabbit Rabbit anti-rat CD4+ T cells CD8 1:1000 (mc) BD Pharmingen, (553038) - Rabbit Rabbit anti-rat CD8+ T cells CD45R 1:1000 (biotinylated mc) BD Biosciences (553085) 20 minutes citrate buffer/MW - - B cells GFAP 1:1000 (pc) DakoCytomation (Z0334) - Goat Goat anti-rabbit Astrocytes Granzyme B 1:400 (pc) Abcam (ab4059) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Effector T cells and NK cells NeuN 1:1600 (mc) Merck/Millipore (MAB377) 20 minutes citrate buffer/MW Goat Goat anti-mouse Neurons TMEV 1:2000 (pc) Department of Pathology, University of Veterinary Medicine Hannover, Germany* - Goat Goat anti-rabbit TMEV capsid protein VP1 β-APP 1:2000 (mc) Merck/Millipore (MAB348) 20 minutes citrate buffer/MW Goat Goat anti-mouse Axonal damage TNF-α 1:50 (mc) Novus NBP2-34303) 20 minutes citrate buffer/MW Goat Goat anti-mouse Inflammatory cytokines producing cells CD11c 1:500 (pc) Invitrogen (PA5-79537) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Activated microglia and infiltrating myeloid or dendritic cells MHC-I 1:200 (pc) Biorbyt (Orb5449) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Presentation of endogenous peptides to CD8+ T cell β-APP = beta-amyloid precursor protein; GFAP = glial fibrillary acidic protein; mc = monoclonal; MW = microwave oven; NeuN = neuronal nuclei; pc = polyclonal; TMEV = Theiler’s murine encephalomyelitis virus; TNF-α = tumor necrosis factor-α; NK cells = natural killer cells; *Kummerfeld et al. [88] Supplementary Table S2. ..
Virus:Article Title: CLEC12A signaling represses protective immune responses and contributes to hippocampal pathology in neurotropic picornavirus infection
Article Snippet: .. Details of antibodies used for immunohistochemistry Primary antibody/dilution Supplier (clone/catalogue number) Pre-treatment Blocking solution Secondary antibody Specificity CD107b 1:200 (mc) AbD Serotec (MCA2293) 20 minutes citrate buffer/MW Rabbit Rabbit anti-rat Macrophages/microglia CD3 1:500(pc) DakoCytomation (A0452) 20 minutes citrate buffer/MW Goat Goat-anti-rabbit T cells CD4 1:2000 (mc) BD Pharmingen, (550280) - Rabbit Rabbit anti-rat CD4+ T cells CD8 1:1000 (mc) BD Pharmingen, (553038) - Rabbit Rabbit anti-rat CD8+ T cells CD45R 1:1000 (biotinylated mc) BD Biosciences (553085) 20 minutes citrate buffer/MW - - B cells GFAP 1:1000 (pc) DakoCytomation (Z0334) - Goat Goat anti-rabbit Astrocytes Granzyme B 1:400 (pc) Abcam (ab4059) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Effector T cells and NK cells NeuN 1:1600 (mc) Merck/Millipore (MAB377) 20 minutes citrate buffer/MW Goat Goat anti-mouse Neurons TMEV 1:2000 (pc) Department of Pathology, University of Veterinary Medicine Hannover, Germany* - Goat Goat anti-rabbit TMEV capsid protein VP1 β-APP 1:2000 (mc) Merck/Millipore (MAB348) 20 minutes citrate buffer/MW Goat Goat anti-mouse Axonal damage TNF-α 1:50 (mc) Novus NBP2-34303) 20 minutes citrate buffer/MW Goat Goat anti-mouse Inflammatory cytokines producing cells CD11c 1:500 (pc) Invitrogen (PA5-79537) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Activated microglia and infiltrating myeloid or dendritic cells MHC-I 1:200 (pc) Biorbyt (Orb5449) 20 minutes citrate buffer/MW Goat Goat anti-rabbit Presentation of endogenous peptides to CD8+ T cell β-APP = beta-amyloid precursor protein; GFAP = glial fibrillary acidic protein; mc = monoclonal; MW = microwave oven; NeuN = neuronal nuclei; pc = polyclonal; TMEV = Theiler’s murine encephalomyelitis virus; TNF-α = tumor necrosis factor-α; NK cells = natural killer cells; *Kummerfeld et al. [88] Supplementary Table S2. ..
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